“Principles of Plant Biotechnology” introduces cellular totipotency, in vitro tissue culture techniques, genetic transformation, recombinant DNA technology, and molecular breeding tools for crop improvement. The material covers organogenesis, somatic embryogenesis, protoplast fusion, Agrobacterium-mediated gene transfer, PCR techniques, and marker-assisted selection (MAS).
Topics Covered
- Concepts, Scope and Historical Background of Biotechnology in Agriculture
- Plant Tissue Culture – History, Laboratory Organization and Sterilization Techniques
- Nutritional Requirements of Explants and Culture Media Formulations (MS Media)
- Cellular Totipotency, Differentiation and Morphogenesis
- Micropropagation Stages, Clonal Fidelity and Commercial Applications
- Organogenesis – Direct and Indirect Shoot and Root Organogenesis
- Somatic Embryogenesis, Synthetic Seeds (Synseeds) and Cryopreservation
- Anther Culture, Pollen Culture and Production of Haploids (Androgenesis)
- Ovule and Embryo Culture – Overcoming Incompatibility Barriers via Embryo Rescue
- Protoplast Isolation, Purification, Culture and Somatic Hybridization (Cybrids)
- Somaclonal Variation – Origin, Screening and Utilization in Crop Breeding
- Recombinant DNA Technology – Restriction Endonucleases, DNA Ligases and Modifying Enzymes
- Cloning Vectors – Plasmids, Bacteriophages, Cosmids, BACs and YACs
- Agrobacterium-Mediated Gene Transfer – Ti Plasmid, T-DNA Structure and Vir Genes
- Direct Gene Transfer Methods – Particle Bombardment (Gene Gun), Electroporation, and Microinjection
- Polymerase Chain Reaction (PCR) – Principles, Variations (RT-PCR, qPCR) and Applications
- DNA Sequencing Technologies (Sanger Sequencing to Next-Generation Sequencing)
- Molecular Markers – Morphological, Biochemical, and Molecular DNA Markers
- Hybridization-based Markers (RFLP) and PCR-based Markers (RAPD, AFLP, SSR/Microsatellites, SNPs)
- Genetic Diversity Analysis and Construction of Molecular Linkage Maps
- Quantitative Trait Locus (QTL) Mapping Concepts and Bi-parental Populations
- Marker-Assisted Selection (MAS) – Foreground, Background Selection and Gene Pyramiding
- Genetically Modified Crops – Transgenic Crops for Insect Resistance (Bt Cotton, Bt Brinjal)
- Herbicide Tolerant Crops (Roundup Ready Crops) and Disease Resistant Transgenics
- Quality Improvement via Genetic Engineering (Golden Rice, Flavr Savr Tomato)
- Biosafety Guidelines, Environmental Risk Assessment, and GEAC Regulations
- Ethical, Social and Intellectual Property Rights (IPRs) in Agricultural Biotechnology
File Details
| Title | Principles of Plant Biotechnology |
| File Name | GPBR311 – Principles of Plant Biotechnology.pdf |
| File Type | PDF Document (.pdf) |
| File Size | 6.84 MB |
| Total Pages | 345 Pages |